• Dorina Rodica CHAMBRE Faculty of Food Engineering, Tourism and Environmental Protection, “Aurel Vlaicu” University, Romania, 2 Elena Dragoi, Arad 310330, Romania
  • Mihaela DOCHIA Research Development Innovation in Natural and Technical Sciences Institute, “Aurel Vlaicu” University, Romania, 2 Elena Dragoi, Arad 310330, Romania
Keywords: hemp fibres, alkaline treatment, enzyme treatments, pectin elimination, FT-IR ATR


The FT-IR ATR spectra of the alkaline and enzymatically treated hemp fibres (1%, 2%, 3% o.w.f. enzyme,
15, 35, 55 minute enzyme action time) were recorded aiming to evaluate the pectin elimination. The relative absorbance
values of the 1732 cm-1 band which might be attributed to the presence of the carboxylic groups (C=O) in pectin were
evaluated. The results showed a diminishing of the pectin amount with increasing concentration (% o.w.f.) and action
time (minutes) of the enzyme mixture. So that, the absorbance recorded at 1732 cm-1 with FT-IR ATR spectroscopy can
quantify removed pectin from the material, thus enabling characterization of proposed biotechnology and monitoring the


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