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Universitatea
„Aurel Vlaicu” din Arad

PROJECT PN-II-ID-0047-2011

Financed by the Romanian National Authority for Scientific Research-ANCS, through the Executive Unit for Financing the Higher Education, Research, Development and Innovation-UEFISCDI

Project director: Prof. Dr. Alina-Diana Zamfir

Contract number: 254/05/11/2011

Budget: 1.500.000 lei

Research Team:

Dr. Alina-Diana Zamfir, PhD
Dr. Eugen Sisu, PhD
Dr. Lizica Mihut, PhD
Dr. Corina Flangea, PhD
Dr. Florina Capitan, PhD
Mirela Sarbu (Galusca), PhD student
Cristina Mosoarca, PhD student
Catalin Schiopu, PhD student

Domain: LS.LIFE SCIENCES

Subdomain: LS2.Genetics, Genomics, Bioinformatics and Systems Biology: genetics, population genetics, molecular genetics, genomics, transcriptomics, proteomics, metabolomics, bioinformatics, computational biology, biostatistics, biologicalmodelling and simulation, system

Main Research Area: LS2_5.Glycomics

Secondary Research Area: LS2_3.Proteomics

Project title in English:

Development of chip-based nanoelectrospray in combination with electron transfer dissociation mass spectrometry for top-down proteomics

Project title in Romanian:

Dezvoltarea tehnicii chip-nanoelectrospray combinata cu spectrometria de masa cu disociere prin transfer de electroni pentru top-down proteomica

Project duration: 05.10.2011-30.09.2016

 

PROJECT SUMMARY

The most popular method for protein de novo identification by mass spectrometry (MS) is based on enzymatic digestion rendering peptides to be analyzed by conventional tandem MS, in the so-called bottom-up approach. Though this method has brought a significant progress to the field, many limitations such as the low throughput and impossibility to characterize in details post-translational modifications, were reported. Therefore, a hot topic in MS-based proteomics is nowadays the development of novel top-down approaches based on intact protein fragmentation by electron transfer dissociation (ETD) technique. In this context, here, an exploratory research project within the IDEAS-2011 call planning to combine for the first time chip-based-MS with ETD and implement it in proteomics and glycoproteomics is proposed. High expertise in MS and life sciences for method application to protein and glycoprotein analysis is a prerequisite for the project. This superior analytical platform, integrating microfluidics technology with ETD-top-down fragmentation could allow efficient structural investigation of proteins in single, high throughput MS experiments. Such a technique may represent a viable alternative to current MS canons in proteomics, with real potential to influence directions of basic research in the field. The novel method and designed analysis protocols are aimed to find application in medicine as rapid and accurate diagnostic tools based on protein molecular fingerprints.

 

PROJECT OBJECTIVES

The objectives of this project are to conceive, introduce in the field of life sciences and develop to the stage of routine use for various biological and clinical samples of a novel bioanalytical platform allowing for the first time de novo protein identification by high-throughput automated top-down proteomics based on ETD sequencing.

EXPECTED ACHIEVEMENTS

  1. first world merging of robotics and microfluidics (chip) with electron transfer dissociation technique and automatic fragmentation by ETD/CID switching;
  2. innovative protocols for high performance microfluidics/MS in combination with ETD top-down fragmentation will be designed, tested and implemented to allow rapid and accurate identification and structural characterization;
  3. The novel, completely automated platform will be applied to structural identification of peptides, glycopeptides, proteins, glycoproteins and other glyco- and bioconjugates which will be infused by robotized chip-nanoESI MS, screened by MS and fragmented by ETD and alternate ETD/CAD;
  4. Validation and testing of the method performance for routine applicability in clinical and biomedical research will be achieved for complex O-glycopeptide mixtures from urine of patients suffering from a recently recognized autosomal recessive disorder, called Schindler’s disease.

 

PROJECT RESULTS 

 IMPLEMENTATION STATUS

STAGE 1 (2011) In the first stage of the project fully automated chip-based nanoESI on a NanoMate robot was coupled to ETD on a high capacity ion trap mass spectrometer (HCT MS). Optimization of the NanoMate HCT MS ETD in a single dissociation stage (ETD MS2) and multi-stage dissociation (ETD MSn) was carried out. 

STAGE 2 (2012) In the second stage of the project, the NanoMate HCT MS ETD platform developed within the first stage was tested and optimized on simple and conjugated peptides, which assumed determination of the aminoacid sequence by ETD fragmentation experiments via automated chip-nanoESI MS infusion.

STAGE 3 (2013) In stage 3 the complete structural analysis of glycopeptides and small proteins was  performed using the developed platform. Thus, in a single experiment with direct automated infusion via chip-nanoESI, determination of the aminoacid sequence and glycosylation site(s) by ETD fragmentation was achieved.

STAGE 4 (2014) In this research stage the complete automation of the chip-nanoESI HCT MS ETD and alternate ETD/CID MSn with automatic switching for top-down analysis of peptides, glycopeptides, proteins, glycoproteins and bioconjugates was achieved. Moreover, complex noncovalently linked protein-glycoconjugate compounds were fully characterized by ETD/CID MSn, with an accurate determination of the protein and the linked glycoconjugate structure as well as the site(s) of the noncovalent attachment.

STAGE 5 (2015) This stage of research was focused on the validation of the top-down platform for highly complex mixtures. Using the newly developed top-down approach, optimization experiments were carried out for ionization, detection, identification and structural characterization of each single species in multicomponent samples. By employing the novel methodology, the expression and structure of glycosylated amino acids and peptides in a fraction separated, pooled and purified from the urine of a patient suffering from Schindler disease were accurately determined.

STAGE 6 (2016) In the final stage of the project the method based on fully automated chip-nanoelectrospray ionization on a NanoMate robot in combination with highly efficient dissociation techniques ETD, CID and ETD/CID was introduced in complex clinical studies. One of the most interesting clinical applications targeted the mapping and structural analysis by tandem MS of ganglioside components in human brain and also in a crude mixture extracted from human neuroblastoma, an extracranial tumor. By our approach, for the first time worldwide, gangliosides from an extracranial tumor, where they exhibit a reduced expression, could be investigated by mass spectrometry. The obtained data demonstrated that the methodology developed within this project provides also a basis for future research oriented towards the elucidation of the biomarker role played by different glycoconjugates and their use in early diagnosis and therapy.

ARTICLES IN IMPACT FACTOR JOURNALS

 

1. Schiopu C, Vukelić Z, Capitan F, Kalanj-Bognar S, Sisu E, Zamfir AD.

Chip-nanoelectrospray quadrupole time-of-flight tandem mass spectrometry of meningioma gangliosides: a preliminary study.

Electrophoresis 2012, 12, 1778-1786; impact factor: 3.30

 

2. Serb AF, Sisu E, Vukelić Z, Zamfir AD.

Profiling and sequencing of gangliosides from human caudate nucleus by chip-nanoelectrospray mass spectrometry.

J. Mass Spectrom. 2012, 47, 1561-1570; impact factor: 3.26

 

3. Flangea C, Schiopu C, Capitan F, Mosoarca C, Manea M, Sisu E, Zamfir AD.

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics.

Cent. Eur. J. Chem. 2013, 11, 25-34; impact factor: 1.073

 

4. Flangea C, Mosoarca C, Cozma C, Galusca M, Przybylski M, Zamfir AD.

Testing the feasibility of fully automated chip-based nanoelectrospray ionization mass spectrometry as a novel tool for rapid diagnosis of Fabry disease.

Electrophoresis 2013, 34, 1572-1580; impact factor: 3.30

 

5. Ghiulai R, Galusca M, Sisu I, Sisu E, Zamfir AD.

High resolution mass spectrometric characterization of amino linked oligosaccharides- a preliminary study.

Cent. Eur. J. Chem., 2013, 8, 1309-1319; impact factor: 1.073

 

6. Flangea C, Petrescu AJ, Seidler DG, Munteanu CV, Zamfir AD.

Identification of an unusually sulfated tetrasaccharide chondroitin/dermatan motif in mouse brain by combining chip-nanoelectrospray multistage MS(2) -MS(4) and high resolution MS.

Electrophoresis 2013, 34, 1581-1592; impact factor: 3.30

 

7. Flangea C, Fabris D, Vukelić Ž, Zamfir AD.

Mass spectrometry of gangliosides from human sensory and motor cortex.

Aus. J. Chem. 2013, 66, 781-790; impact factor: 2.34

 

8. Zamfir AD, Fabris D, Capitan F, Munteanu C, Vukelić Ž, Flangea C.

Profiling and sequence analysis of gangliosides in human astrocytoma by high resolution mass spectrometry

Anal. Bioanal. Chem. 2013, 405, 7321-7335; impact factor: 3.77

9. Sarbu (Galusca) M, Ghiulai RM, Zamfir AD.
Recent developments and applications of electron transfer dissociation mass spectrometry in proteomics.
Amino Acids
2014, 46, 1625-1634; impact factor: 3.65

10. Nikolovska K, Renke JK, Jungmann O, Grobe K, Iozzo RV, Zamfir AD, Seidler DG.
A decorin-deficient matrix affects skin chondroitin/dermatan sulfate levels and keratinocyte function.
Matrix Biol
.,  2014, 35, 91-102; impact factor: 3.64

 11. Ghiulai RM, Sarbu (Galusca) M, Vukelić Ž, Ilie C, Zamfir AD. Early stage fetal neocortex exhibits a complex ganglioside profile as revealed by high resolution tandem mass spectrometry.
Glycoconj J
. 2014, 31, 231-245; impact factor: 1.94 

 12. Sarbu M, Robu A, Peter-Katalinic J, Zamfir AD.
Automated chip-nanoelectrospray mass spectrometry for glycourinomics in Schindler disease type I.
Carbohydr. Res.
2014, 398, 90-100; impact factor: 2.04

13. Ghiulai RM, Sarbu M, Ilie C, Zamfir AD.
A straightforward electrospray ionization high resolution mass spectrometry method for underivatized long chain polysaccharides Cent. Eur. J. Chem-Open Chem. 2015, 13, 32-41; impact factor: 1.33 

14. Sârbu M, Zhu F, Peter-Katalinić  J, Clemmer DE,  Zamfir AD.
Application of ion mobility tandem mass spectrometry to compositional and structural analysis of glycopeptides extracted from the urine of a patient diagnosed with Schindler disease. Rapid Commun. Mass Spectrom. 2015, 29, 1929-1937; impact factor: 2.25

15. Capitan F, Robu AC, Schiopu C, Ilie C, Chait BT, Przybylski M, Zamfir AD.
β-Lactoglobulin detected in human milk forms noncovalent complexes with maltooligosaccharides as revealed by chip-nanoelectrospray high-resolution tandem mass spectrometry. Amino Acids, 2015, 47, 2399-2407; impact factor: 3.29  

16. Sârbu M, Munteanu CVA, Dehelean L, Petrescu AJ, Peter-Katalinić J, Zamfir AD.
Identification and structural characterization of novel O- and N-glycoforms in the urine of a Schindler disease patient by Orbitrap mass spectrometry. J. Mass Spectrom. 2015,  50, 1044–1056; impact factor: 2.37

17. Robu AC, Popescu L, Munteanu CVA, Seidler DG, Zamfir AD.Orbitrap mass spectrometry characterization of hybrid chondroitin/dermatan sulfate hexasaccharide domains expressed in brain.
Anal. Biochem
. 2015, 485, 122-131; impact factor: 2,21 

18. Căpitan F, Robu A, Popescu L, Flangea C, Vukelić Ž, Zamfir AD.
B subunit monomers of Cholerae toxin bind G1 ganglioside class as revealed by chip-nanoelectrospray multistage mass spectrometry.

J. Carbohydr. Chem.
2015, 34, 388-408; impact factor: 1.18

19. Sarbu (Galusca) M, Robu A, Ghiulai R, Vukelic Z, Clemmer DE, Zamfir AD
Electrospray ionization ion mobility mass spectrometry of human brain gangliosides.  Anal. Chem. 2016, 88, 5166-5178; impact factor: 5.62

20. Robu A, Schiopu C, Capitan F, Zamfir AD. Mass spectrometry of gangliosides in extracranial tumors: Application to adrenal neuroblastoma. Anal. Biochem. 2016, 509, 1-11; impact factor: 2.20

21. Zamfir AD
Applications of capillary electrophoresis electrospray ionization mass spectrometry in glycosaminoglycan analysis. Electrophoresis 2016, 37, 973-986;  impact factor: 3.00

BOOK CHAPTERS

1. Zamfir AD, Flangea C, Serb A, Sisu E, Zagrean L, Rizzi A, Seidler DG.

Brain chondroitin/dermatan sulfate, from cerebral tissue to fine structure: extraction, preparation, and fully automated chip-electrospray mass spectrometric analysis.

Methods Mol. Biol.2012, 836, 145-159; ©Springer

 

2. Alamae T, Visnapuu T, Mardo K, Mae A, Zamfir AD.

Levansucrases of Pseudomonas bacteria: novel approaches for protein expression,  assay of enzymes, fructooligosaccharides and heterooligofructans.

Carbohydr. Chem. 2012, 38, 176–191; ©The Royal Society of Chemistry

 

3. Zamfir AD, Flangea C, Serb A, Zagrean AM, Rizzi AM, Sisu E.

Separation and identification of glycoforms by capillary electrophoresis with electrospray ionization mass spectrometric detection.

Methods Mol. Biol.2013, 951, 145-169; ©Springer

4. Zamfir AD. Neurological analyses: focus on gangliosides and mass spectrometry.
Adv. Exp. Med. Biol.
2014, 806, 153-204; © Springer

5. Zamfir AD. Nanochips for mass spectrometry and applications in biomedical research.
in: "Research horizons of nanosystems: structure, properties and interactions"
2014, 386-443, ISBN: 978-1926895-90-1 ©Apple Academics Press, Ontario-New Jersey, Canada-USA

6. Sarbu M, Zamfir AD.

Nanofluidics-based mass spectrometry. Applications for biomarker discovery in lysosomal storage diseases .

in: "Nanoparticles: Promises and Risks", 2015, 137-165, ISBN: 978-3-319-11727-0 © Springer

 

CONFERENCE PRESENTATIONS

  1. C. Flangea, C. Schiopu, F. Capitan, C. Mosoarca, M. Manea, E. Sisu, A.D. Zamfir, A novel platform for top-down proteomics: chip-based nanoelectrospray combined with electron transfer dissociation mass spectrometry. Joint Conference of Polish Mass Spectrometry Society and German Mass Spectrometry Society, 4-7 March 2012, Poznan, Poland
  2. M.I. Iurascu, C. Cozma, M. Galusca, M. Przybylski, Oligomerisation studies of modified A-beta peptides using mass spectrometry. Joint Conference of Polish Mass Spectrometry Society and German Mass Spectrometry Society, 4-7 March 2012, Poznan, Poland
  3. C. Cozma, M.I. Iurascu, M. Galusca, S. Maeser, M. Przybylski, Clinical diagnostic in dry blood spots by fluorometry and multiple reaction monitoring mass spectrometry. Joint Conference of Polish Mass Spectrometry Society and German Mass Spectrometry Society, 4-7 March 2012, Poznan, Poland
  4. C. Mosoarca, A.D. Zamfir, Chip-based nanoelectrospray mass spectrometry of fructooligosaccharides produced by levansucrases from Pseudomonas Syringae Pv. Tomato and P. Chlororaphis Subs. P. Aurantiaca. Joint Conference of Polish Mass Spectrometry Society and German Mass Spectrometry Society, 4-7 March 2012, Poznan, Poland
  5. C. Flangea, M. Galusca, M. Manea, A.D. Zamfir, High throughput top-down proteomics by chip-based nanoelectrospray in combination with electron transfer dissociation mass spectrometry. 4th International Congress and the 30th Annual Session of the Romanian Society for Cell Biology, 13-17 June 2012, Satu Mare, Romania
  6. C. Flangea, M. Galusca, A.D. Zamfir, Rapid diagnostic by fully automated chip-based nanoelectrospray ionization mass spectrometry. 4th International Congress and the 30th Annual Session of the Romanian Society for Cell Biology, 13-17 June 2012, Satu Mare, Romania
  7. C. Flangea, C. Mosoarca, M. Manea, C. Schiopu, E. Sisu, A.D. Zamfir, Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics. International Mass Spectrometry Conference, 11-22 September 2012, Kyoto, Japan
  8. M. Galusca, C. Flangea, C. Mosoarca, C. Cozma, A.D. Zamfir, Chip-based electrospray ionization tandem mass spectrometry a novel tool for rapid diagnostic of Fabry disease. International Mass Spectrometry Conference, 11-22 September 2012, Kyoto, Japan
  9. F. Capitan, C. Schiopu, C. Mosoarca, C. Flangea,  C. Ilie, I. Velea, E. Sisu, A.D. Zamfir, Compositional and structural analysis of gangliosides in human fetal cerebellum by chip electrospray mass spectrometry. International Symposium Research and Education in Innovation Era, 8-9 November 2012, Arad, Romania
  10. M. Galusca, C. Flangea, C. Mosoarca, C. Cozma, AD. Zamfir, Chip-based electrospray ionization tandem mass spectrometry a novel tool for rapid diagnostic. International Symposium Research and Education in Innovation Era, 8-9 November 2012, Arad, Romania
  11. F. Capitan, C. Schiopu, C. Flangea, Ž. Vukelić, A.D. Zamfir, Mapping and sequencing of gangliosides in human astrocytoma by Orbitrap mass spectrometry. 24th Mass Spectrometry Forum, 19-23 February, 2013, Vienna, Austria
  12. M. Galusca, C. Flangea, C. Mosoarca, C. Cozma, AD. Zamfir, Rapid diagnosis of Fabry disease by a novel method based on automated chip-nanoelectrospray ionization mass spectrometry. 24th Mass Spectrometry Forum, 19-23 February 2013, Vienna, Austria
  13. A.D. Zamfir, C. Mosoarca, C. Flangea, C. Cozma, I. Malaescu, M. Przybylski, Fully automated chip-nanoelectrospray combined with collision induced and electron transfer dissociation. 46th German Society for Mass Spectrometry Conference, 10-14 March 2013, Berlin, Germany
  14. F. Capitan, C. Flangea, A. Petrescu, C. Munteanu, A.D. Zamfir, Introducing Orbitrap mass spectrometry for ganglioside biomarker discovery in human brain cancer: application to astrocytoma, 46th German Society for Mass Spectrometry Conference, 10-14 March 2013, Berlin, Germany
  15. C. Mosoarca, C. Flangea, M. Galusca, A.D. Zamfir, Rapid diagnosis through molecular markers identified by advanced mass spectrometry. 5th International Congress and the 31th Annual Session of the Romanian Society for Cell Biology, 5-9 June 2013, Timisoara, Romania
  16. M. Galusca (Sarbu), C. Flangea, C. Mosoarca, C. Cozma, A.D. Zamfir, Fabry disease-Rapid diagnosis by chip-based nanoelectrospray ionization tandem mass spectrometry. 2nd Middle Eastern and Mediterranean Sea Region Countries Mass Spectrometry Conference, June 30 - July 4, 2013, Siena, Italy
  17. C. Mosoarca, T. Visnapuu, T. Alamae, A.D Zamfir, Chip-based mass spectrometry of oligosaccharides produced by levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca.  2nd Biotechnology World Congress, 10-13 February 2014, Dubai, United Arab Emirates
  18. C. Mosoarca, L. Lupu, I. Malaescu, A.D. Zamfir, Top-down analysis by electron transfer/collision induced dissociation of noncovalent protein-ganglioside complexes.  25th Mass Spectrometry Forum, 17-19 February 2014, Vienna, Austria
  19. M. Sarbu, A. Robu, R. Ghiulai, J. Peter-Katalinic, A.D. Zamfir, Applications of chip-based nanoelectrospray MS to lysosomal storage diseases: Schindler disease. 25th Mass Spectrometry Forum, 17-19 February 2014, Vienna, Austria
  20. M. Sarbu, R. Ghiulai, A.D. Zamfir, A nanoESI-QTOF MS approach for screening and sequencing of underivatized dextran chains containing up to 35 glucose repeats.  20th International Mass Spectrometry Conference, 24-29 August 2014, Geneva, Switzerland 
  21. C. Mosoarca, A.D. Zamfir, Introduction of chip-based nanoelectrospray mass spectrometry with electron transfer/collision induced dissociation in glycoproteomics.  20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary
  22. A.D. Zamfir, M. Sarbu, R. Ghiulai, Nanoelectrospray quadrupole time of flight tandem mass spectrometric analysis of long chain polysaccharides. 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary
  23. M.Sarbu, R.M. Ghiulai, A.D. Zamfir, Nanoelectrospray quadrupole time-of-flight tandem mass spectrometric analysis of long chain polysaccharides. 5th International Symposium. Research and Education in Innovation Era, 5-6 November 2014, Arad, Romania
  24. A C. Robu, M. Sarbu, C. C. Darie, J. Peter-Katalinic, AD. Zamfir, Identification of the O-glycoforms expressed in the urine of a patient suffering from Schindler disease using chip-based nanoelectrospray tandem mass spectrometry. Eastern Analytical Symposium and Exhibition, 17-19 November, Somerset, New Jersey, USA
  25. L. Lupu, Z. Vukelic, C.C. Darie, AD Zamfir, Glycan biomarker discovery in human gliosarcoma by chip-based nanoelectrospray tandem mass spectrometry. Eastern Analytical Symposium and Exhibition, 17-19 November, Somerset, New Jersey, USA
  26. C. Mosoarca, I. Malaescu, Ž. Vukelić, AD. Zamfir, A. Ercuta, Introducing 2D numerical analysis in modelling of complex carbohydrate mass spectra. TIM-FIZ 2014 Physics without frontiers, International Physics Conference, 20-22, November 2014, Timisoara, Romania
  27. M. Sarbu, A. Robu, I. Malaescu, AD. Zamfir, Chip-based mass spectrometry for Schindler disease type I: detailed analysis of a complex glycopeptide fraction from patient urine. TIM-FIZ 2014 Physics without frontiers, International Physics Conference, 20-22, November 2014, Timisoara, Romania
  28. M. Sârbu, C.V.A. Munteanu, A, Petrescu, J. Peter-Katalinić, A.D. Zamfir, Novel glycopeptide structures highlighted by Orbitrap MS and MS2 analysis in the urine of a Schindler disease patient. 26th Mass Spectrometry Forum, 17-19 February, 2015, Vienna, Austria
  29. C.V.A. Munteanu, L. Popescu, D. G. Seidler, A. D. Zamfir, High sensitivity Orbitrap mass spectrometry analysis of neural chondroitin/dermatan sulfate domains. 26th Mass Spectrometry Forum, 17-19 February, 2015, Vienna, Austria
  30. R. M. Ghiulai, Sârbu M., Vukelić Ž., C. Ilie, A. D. Zamfir, High resolution mass spectrometric analysis of complex ganglioside profile typical to early stage fetal neocortex. 26th Mass Spectrometry Forum, 17-19 February, 2015, Vienna, Austria
  31. A.C. Robu, R. Aslebagh, K.F. Arcaro, C.C. Darie, A.D. Zamfir, Discovering breast cancer biomarkers in human milk by advanced proteomics methods based on mass spectrometry. 26th Mass Spectrometry Forum, 17-19 February, 2015, Vienna, Austria
  32. M. Sârbu, F. Zhu, J. Peter-Katalinić, D.E. Clemmer, M. Przybylski, A.D. Zamfir, Introducing ion mobility mass spectrometry in glycomics of Schindler disease. 48th German Society for Mass Spectrometry Conference (Deutschen Gesellschaft für Massenspektrometrie-DGMS), 1-4 March 2015, Wuppertal, Germany
  33. A.C. Robu, R. Aslebagh, C.C. Darie, B. Chait, M. Przybylski, A.D. Zamfir, Development of advanced proteomics methods based on mass spectrometry for breast cancer biomarker discovery in human milk. 48th German Society for Mass Spectrometry Conference (Deutschen Gesellschaft für Massenspektrometrie-DGMS), 1-4 March 2015, Wuppertal, Germany
  34. A.C. Robu, R. Aslebagh, C.C. Darie, B. Chait, M. Przybylski, A.D. Zamfir, Proteomics methods based on gel electrophoresis and nanoLC-MS/MS for discovering newbreast cancer biomarkers in human milk. 1st International Symposium Biomolecular Recognition and Mass Spectrometry, 5-7 March 2015, Rüsselsheim, Germany
  35. L. Popescu, A.C. Robu, F. D. Munteanu, D. G. Seidler, A.D. Zamfir, Compositional  and structural analysis of brain chondroitin/dermatan sulfate hexasaccharides by Orbitrap mass spectrometry. 3rd Mediterranean Sea Region Countries Mass Spectrometry Workshop, 28 June- 2 July 2015, Athens, Greece
  36. A.C. Robu, F. Capitan, C. Schiopu, B.T. Chait, A.D. Zamfir, Assessment of the noncovalent interaction between β-lactoglobulin detected in human milk and maltooligosaccharides by chip-nanoelectrospray high resolution tandem mass spectrometry. 3rd Mediterranean Sea Region Countries Mass Spectrometry Workshop, 28 June- 2 July 2015, Athens, Greece
  37. A.D. Zamfir, Clinical applications of chip-based mass spectrometry. 25th Anniversary Conference of the Romanian Society of Biochemistry and Molecular Biology, 17-18 September, Bucharest, Romania
  38. A.D. Zamfir, A decade of GlycoRizzi-based Austrian-Romanian mass spectrometry. Bioanalysis Symposium and Honorary Coloquium, 5-6 November 2015, Vienna, Austria
  39. A. Robu, L. Popescu, F. Capitan, A.D. Zamfir,  ChipESI MSn of Ctb5 monomers-G1 ganglioside complexes. 27th Mass Spectrometry Forum 23-25 February, 2016, Vienna, Austria
  40. A.D. Zamfir, A. Robu, F. Capitan, C. Schiopu,     Neuroblastoma ganglioside analysis by chip-ESI MS. 27th Mass Spectrometry Forum 23-25 February, 2016, Vienna, Austria
  41. M. Sarbu (Galusca), A. Robu, M. Trion, D. Clemmer, A.D. Zamfir, IMS MS and MS/MS of human brain gangliosides. 27th Mass Spectrometry Forum 23-25 February, 2016, Vienna, Austria
  42. A.D. Zamfir, A. Robu, F. Capitan, C. Schiopu, Microfluidics-mass spectrometry of brain tumor gangliosides. 13th International Conference on Nanosciences & Nanotechnologies, 5-9 July, Thessaloniki, Greece
  43. A. Robu, L. Popescu, F. D. Munteanu, A.D. Zamfir, Glycomics of GAG oligosaccharides by high-performance mass spectrometry. 13th International Conference on Nanosciences & Nanotechnologies, 5-9 July, Thessaloniki, Greece
  44. M. Sarbu (Galusca), F.D. Munteanu, D. E. Clemmer, A.D. Zamfir, Ion mobility mass spectrometry of glycolipids. 13th International Conference on Nanosciences & Nanotechnologies, 5-9 July, Thessaloniki, Greece

 

 

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